Vitamin D, 25-Hydroxy is drawn in a Serum tube at Quest, a Red-top tube or gel-barrier tube at LabCorp, and a SST Tube at BioReference.

Lab comparison

Vitamin D, 25-Hydroxy: Quest vs LabCorp vs BioReference, tube, code & requirements

. This page shows collection requirements for all three major reference labs in one view, so you don’t have to cross-check three portals.

Always verify against your lab’s current collection manual before drawing.

Every value on this page is verified by a nurse practitioner in active clinical practice, not scraped from an automated source.

View one lab

RequirementQuest DiagnosticsLabCorpBioReference
Test code173060819500286-5
Primary tubeSerum tubeRed-top tube or gel-barrier tubeSST Tube
Specimen type0.8 mL serumSerum1 mL SST Tube
Patient prepFasting preferred, but not requiredFasting preferred, but not requiredFasting preferred, but not required
Minimum volume
Minimum Volume
0.5 mL
Minimum Volume
0.3 mL (Note: This volume does not allow for repeat testing.)
Qty
1

Frequently asked questions

What is the Quest Diagnostics order code for Vitamin D, 25-Hydroxy?

The Quest Diagnostics order code for Vitamin D, 25-Hydroxy is 17306.

What tube color does LabCorp use for Vitamin D, 25-Hydroxy?

LabCorp uses a Red-top tube or gel-barrier tube for Vitamin D, 25-Hydroxy.

Does Vitamin D, 25-Hydroxy require fasting?

Vitamin D, 25-Hydroxy patient prep: Fasting preferred, but not required. Always confirm with the ordering lab before the draw.

Data provenance

Source: Quest Diagnostics specimen collection manual, LabCorp directory of services, and BioReference laboratory collection guide.

Verified by: a licensed nurse practitioner in active clinical practice. Values are reviewed against each lab’s published collection requirements. Always confirm against your lab’s current manual before drawing.

Quest verified by: Mandi Boss B Franklin, Jul 4, 2026

LabCorp verified by: Mandi Boss B Franklin, Jul 4, 2026

BioReference verified by: Mandi Boss B Franklin, Jul 8, 2026

Need the full lookup, not just a comparison?

No login · Zero PHI · Clinician-verified · Quest + LabCorp + BioReference.

Try a lookup

More test comparisons

Related: why lab specimens get rejected, and how to prevent it

← All comparison guides